A TIME-LAPSE STUDY OF EFFECTS OF ANTICELLULAR ANTIBODY ON MEMBRANE MOBILITY AND PHAGOCYTIC ACTIVITY OF HELA CELLS

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Abstract
These time-lapse studies demonstrate that intact anticellular antibody inhibited the movement of cell surface and that the extend of inhibition was dependent upon the concentration of antibody in the culture. The inhibition of membrane mobility was associated with inhibition of phagocytosis, and these effects were directly related. Univalent antibody fragments did not alter membrane mobility or phagocytic activity. These observations indicate that anticellular antibody inhibited the phagocytic activity of HeLa cells by immobilizing the plasma membrane. The previously published observation that antibody agglutinates the cell membrane and the above observations with univalent fragments indicate that immobilization of the cell periphery may be due to agglutination of the plasma membrane by intact antibody.