Monoclonal Antibody (F5) to Human Prostate Antigen

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Abstract
Hybridoma culture F5 was developed which secretes monoclonal antibody (McAb) directed to an epitope of a prostatic glycoprotein of [human] MW 34 kD [kilodalton] (Prostate Antigen, PA). Tissue levels of PA were evaluated using a competitive-binding enzyme-immunoassay based upon the inhibition of McAb binding activity to purified antigen. Results indicated the specific occurrence of high antigen concentrations in extracts prepared from prostatic tissues. The antigenicity of epitope F5 is resistant to tissue fixation and embedding protocols, and was demonstrated upon immunoperoxidase staining procedures. McAb F5 possesses a singular specificity towards prostatic epithelial cells. Other tissues, whether normal or cancerous, fail to express this determinant. Specimens examined included epithelial and nonepithelial tissues along with a panel of carcinomas and sarcomas. The antibody was able to detect tumor cells at extra-prostatic sites and represents a powerful probe for the detection and differential diagnosis of metastatic cancer of the prostate.