ISOZYME CHARACTERIZATION OF KENTUCKY BLUEGRASS CULTIVARS
- 1 October 1985
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Plant Science
- Vol. 65 (4) , 985-994
- https://doi.org/10.4141/cjps85-126
Abstract
Twenty-two cultivars of Kentucky bluegrass (Poa pratensis L.) were examined for their glucose phosphate isomerase, triose phosphate isomerase, phosphoglucomutase, malate dehydrogenase, 6-phosphogluconate dehydrogenase and alpha-naphthyl esterase phenotypes. Polymorphism was observed in each isozyme system, and the first four systems were characterized for variation among and within the cultivars. Sixteen of the 22 cultivars could be uniquely identified by selecting isozymes which displayed clearly resolved bands and exhibited little or no intracultivar polymorphism. Analysis could be performed on single plants, permitting estimation of percent contamination should seed from different cultivars be mixed. Thus horizontal starch gel electrophoresis in combination with specific isozyme assays appears to offer a simple system for cultivar identification of Kentucky bluegrass seed.Key words: Cultivar identification, electrophoresis, isozymes, bluegrass (Kentucky)This publication has 0 references indexed in Scilit: