Immunochemical characterization of the modulator protein of the ATP,Mg‐dependent protein phosphatase
- 9 May 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 232 (1) , 167-171
- https://doi.org/10.1016/0014-5793(88)80410-1
Abstract
Polyclonal antibodies raised against the modulator protein of the ATP,Mg‐dependent protein phosphatase completely neutralize all known properties of the purified modulator: inhibition or inactivation of the phosphatase catalytic subunit as well as the kinase FA‐mediated activation of the ATP,Mg‐dependent phosphatase. They do not cross‐react with phosphoinhibitor‐1 or the phosphatase catalytic subunit. Direct analysis of boiled or unboiled skeletal muscle extracts by Western blotting reveals a 32 kDa polypeptide corresponding to the modulator protein as the most dominant protein staining band.Keywords
This publication has 15 references indexed in Scilit:
- Isolation and characterization of two 70 kDa modulator-complexes from rabbit skeletal muscleBiochemical and Biophysical Research Communications, 1986
- The protein phosphatases involved in cellular regulationEuropean Journal of Biochemistry, 1986
- Phosphoprotein phosphatase inhibitor‐2 is phosphorylated at both serine and threonine residues in mouse diaphragmFEBS Letters, 1985
- The catalytic subunits of protein phosphatase‐1 and protein phosphatase 2A are distinct gene productsEuropean Journal of Biochemistry, 1984
- Regulation of ATP—Mg-Dependent Protein PhosphataseCurrent Topics in Cellular Regulation, 1984
- The heat labile phosphatase modulator (inhibitor-2) complex from rabbit skeletal muscleBiochemical and Biophysical Research Communications, 1983
- Complete Primary Structure of Protein Phosphatase Inhibitor‐1 from Rabbit Skeletal MuscleEuropean Journal of Biochemistry, 1982
- A simplified procedure for the purification of the protein phosphatase modulator (inhibitor‐2) from rabbit skeletal muscleFEBS Letters, 1981
- [43] Preparation of homogeneous cyclic AMP-dependent protein kinase(s) and its subunits from rabbit skeletal musclePublished by Elsevier ,1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970