In vivo studies on the incorporation of microinjected acidic proteins of the large ribosomal subunit from Escherichia coli and Artemia salina into oocyte ribosomes from Xenopus laevis
- 18 September 1979
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 18 (19) , 4144-4147
- https://doi.org/10.1021/bi00586a014
Abstract
[3H]-labeled acidic proteins from the large ribosomal subunit of A. salina or E. coli were microinjected into cytoplasm of stage IV/V oocytes from X. laevis. eL12 from the large ribosomal subunit of A. salina but not L7/L12 or L7/L12-L10 from E. coli is specifically incorporated into 60S ribosomal subunits of oocytes. This incorporation is not significantly inhibited by actinomycin D. Incorporation of eL12 into the 60S subunits occurs in enucleated oocytes, suggesting that active ribosomal RNA synthesis and ribosome assembly as well are not prerequired for this reaction. Apparently the incorporation proceeds via an exchange reaction between a free cytoplasmic pool of eL12 and ribosomal eL12.Keywords
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