Improved calcium imaging in transgenic mice expressing a troponin C–based biosensor
- 28 January 2007
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 4 (2) , 127-129
- https://doi.org/10.1038/nmeth1009
Abstract
Fluorescent Ca2+ indicator proteins (FCIPs) are attractive tools for studying Ca2+ dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca2+ sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca2+ imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.Keywords
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