Identification of negative-strand complements to cytochrome oxidase subunit III RNA in Trypanosoma brucei.
- 1 December 1991
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (23) , 10671-10675
- https://doi.org/10.1073/pnas.88.23.10671
Abstract
A substantial amount of cytochrome oxidase subunit III (COIII) mRNA continues to be synthesized de novo in Trypanosoma brucei in the presence of actinomycin D, presumably by a DNA-independent transcription process. We describe the identification of negative-strand COIII RNA molecules, characterization of their termini, and the detection of RNA-dependent RNA polymerase activity. Three lines of evidence for the existence of negative-strand COIII RNA are presented: (i) hybridization with oligonucleotide probes with the same polarity as mRNA after preliminary enrichment for putative negative-strand RNA by affinity purification; (ii) cloning and sequencing of negative-strand complements for the unedited, edited, and partially edited COIII RNA; and (iii) exact correspondence of the terminal sequences of the putative negative-strand RNA molecules to the ends of COIII RNA. The presence of negative-strand complements of COIII RNA is consistent with the notion that a significant amount of mRNA in T. brucei is synthesized by an RNA-dependent RNA polymerase with negative-strand RNA as an intermediate template.Keywords
This publication has 11 references indexed in Scilit:
- RNA editing in mitochondrial mRNA of trypanosomatidsTrends in Biochemical Sciences, 1991
- Guide RNAs in kinetoplastid mitochondria have a nonencoded 3′ oligo(U) tail involved in recognition of the preedited regionCell, 1990
- An extensively edited mitochondrial transcript in kinetoplastids encodes a protein homologous to ATPase subunit 6Cell, 1990
- Uncoupling of the synthesis of edited and unedited COIII RNA in Trypanosoma bruceiNature, 1990
- A model for RNA editing in kinetoplastid mitochondria: RNA molecules transcribed from maxicircle DNA provide the edited informationPublished by Elsevier ,1990
- Trans splicing in trypanosomes — archaism or adaptation?Trends in Genetics, 1989
- Characterization of cytochrome c oxidase III transcripts that are edited only in the 3′ regionCell, 1988
- Extensive editing of the cytochrome c oxidase III transcript in Trypanosoma bruceiCell, 1988
- Editing of kinetoplastid mitochondrial mRNAs by uridine addition and deletion generates conserved amino acid sequences and AUG initiation codonsCell, 1988
- DISCONTINUOUS TRANSCRIPTION AND ANTIGENIC VARIATION IN TRYPANOSOMESAnnual Review of Biochemistry, 1986