Selective loss of beta 2-microglobulin mRNA in human colon carcinoma.
Open Access
- 1 January 1989
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 169 (1) , 309-314
- https://doi.org/10.1084/jem.169.1.309
Abstract
Expression of MHC class I antigens requires the intracellular assembly of HLA-A,B,C H chains and beta 2-microglobulin (beta 2m). We have investigated the expression of free H chain, beta 2m, and their mRNAs in tissue sections of colon carcinomas that were defective for the native two-chain molecule. In all of these tumors, beta 2m protein and mRNA were found to be completely absent from the neoplastic cells, whereas free H chains and their respective mRNAs were present in abundance in the tumor cell cytoplasm. The selective abrogation of beta 2m expression represents a unique mechanism leading to a complete loss of class I antigen surface expression in vivo.This publication has 21 references indexed in Scilit:
- Allele and locus‐specific differences in cell surface expression and the association of HLA class I heavy chain with β2‐microglobulin: differential effects of inhibition of glycosylation on class I subunit associationEuropean Journal of Immunology, 1988
- Defective expression of MHC class I antigens is frequent in B‐cell lymphomas of high‐grade malignancyInternational Journal of Cancer, 1987
- [34] In Situ hybridization for the study of gene expression in the brainPublished by Elsevier ,1986
- Markedly decreased expression of class I histocompatibility antigens, protein, and mRNA in human small-cell lung cancer.The Journal of Experimental Medicine, 1985
- Structure of wild-type and mutant mouse β2-microglobulin genesCell, 1982
- Epitopic analysis of detergent-solubilized HLA molecules by solid-phase radioimmunoassayJournal of Immunological Methods, 1981
- Major histocompatibility antigens: The human (HLA-A,-B,-C) and murine (H-2K, H-2D) class I moleculesCell, 1981
- Characterization of a monoclonal anti‐β2‐microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigensEuropean Journal of Immunology, 1979
- MHC-Restricted Cytotoxic T Cells: Studies on the Biological Role of Polymorphic Major Transplantation Antigens Determining T-Cell Restriction-Specificity, Function, and ResponsivenessPublished by Elsevier ,1979
- Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysisCell, 1978