In Vitro Proliferation of Cells Derived From Adult Human β-Cells Revealed By Cell-Lineage Tracing
- 1 June 2008
- journal article
- Published by American Diabetes Association in Diabetes
- Vol. 57 (6) , 1575-1583
- https://doi.org/10.2337/db07-1283
Abstract
OBJECTIVE— Expansion of insulin-producing β-cells from adult human islets could alleviate donor shortage for cell-replacement therapy of diabetes. A major obstacle to development of effective expansion protocols is the rapid loss of β-cell markers in the cultured cells. Here, we report a genetic cell-lineage tracing approach for following the fate of cultured β-cells.Keywords
This publication has 21 references indexed in Scilit:
- Human Islet-Derived Precursor Cells Are Mesenchymal Stromal Cells That Differentiate and Mature to Hormone-Expressing Cells In VivoThe International Journal of Cell Cloning, 2007
- Generation and expansion of multipotent mesenchymal progenitor cells from cultured human pancreatic isletsCell Death & Differentiation, 2007
- Endocrine precursor cells from mouse islets are not generated by epithelial-to-mesenchymal transition of mature beta cellsMolecular and Cellular Endocrinology, 2007
- Lineage Tracing Evidence for In Vitro Dedifferentiation but Rare Proliferation of Mouse Pancreatic β-CellsDiabetes, 2007
- No Evidence for Mouse Pancreatic β-Cell Epithelial-Mesenchymal Transition In VitroDiabetes, 2007
- Islet-Derived Fibroblast-Like Cells Are Not Derived via Epithelial-Mesenchymal Transition From Pdx-1 or Insulin-Positive CellsDiabetes, 2007
- Expansion and redifferentiation of adult human pancreatic islet cellsBiochemical and Biophysical Research Communications, 2006
- Redifferentiation of insulin-secreting cells after in vitro expansion of adult human pancreatic islet tissueBiochemical and Biophysical Research Communications, 2005
- Adult pancreatic β-cells are formed by self-duplication rather than stem-cell differentiationNature, 2004
- Ultrastructural studies of the ontogeny of fetal human and procine endocrine pancreas, with special reference to colocalization of the four major islet hormonesDevelopmental Biology, 1992