A 45 000 Molecular Weight Human Renin Precursor is Synthesized in a Cell-Free Translation System

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Abstract
1. Human kidney mRNA species were isolated and fractionated through a continuous sucrose gradient ultracentrifugation. 2. mRNA fractions were translated by using a rabbit reticulocyte lysate and [35S]metbionine as tracer. Double immunoprecipitation was carried out with highly specific anti-human renin and anti-rabbit γ-globulin antisera. 3. A 15S mRNA has been found to direct synthesis of a 45 000 molecular weight protein immunoprecipitable with anti-human renin. This protein is considered to be the ultimate precursor of renin (preprorenin).