Procedure for the isolation of lipases produced by Pseudomonas species and Achromobacter lipolyticum
- 1 April 1978
- journal article
- Published by Oxford University Press (OUP) in International Journal of Food Science & Technology
- Vol. 13 (2) , 129-136
- https://doi.org/10.1111/j.1365-2621.1978.tb00786.x
Abstract
Summary: The extracellular lipase systems from three Pseudomonas species and a strain of Achromobacter lipolyticum were concentrated and partially purified using a D.D.S. plate‐and‐frame ultrafiltration unit. Recoveries ranging from 75–97% were reported for the pseudomonad enzyme systems with increases in specific activity of 18–40 times. The low yields obtained for Achromobacter lipolyticum preparations were concluded to be due to the loss of a loosely bound ion or low molecular weight compound necessary for optimum activity.This publication has 16 references indexed in Scilit:
- Preparation of gram quantities of a purified R-factor-mediated penicillinase from Escherichia coli strain W3310Biochemical Journal, 1972
- New Observations on the Substrate Specificity of Cathepsin C (Dipeptidyl Aminopeptidase I)Journal of Biological Chemistry, 1969
- The Purification and Characterization of L-Histidine Ammonia-lyase (Pseudomonas)Journal of Biological Chemistry, 1969
- Lipase from Pseudomonas fragi. I. Purification of the Enzyme1Applied Microbiology, 1969
- Enzyme and bacteriophage concentration by membrane filtrationAnalytical Biochemistry, 1968
- Isolation and characterization of the potato α-1,4-glucan-α-1,4-glucan 6-glucosyltransferaseBiochemistry, 1968
- Protein Solutions: Concentration by a Rapid MethodScience, 1965
- PURIFICATION AND PROPERTIES OF THE EXOPENICILLINASE FROM STAPHYLOCOCCUS AUREUSBiochemical Journal, 1963
- LIPOLYTIC ACTIVITY OF MICROORGANISMS AT LOW AND INTERMEDIATE TEMPERATURES. I. ACTION OF PSEUDOMONAS FLUORESCENS ON LARDJournal of Food Science, 1960
- Protein Determination of Large Numbers of SamplesAnalytical Chemistry, 1959