Lymphokine Production by C3b-Stimulated B Cells

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Abstract
Trypsin cleavage of guinea pig C3 (60 sec, 20°C) yields a fragment which stimulates guinea pig lymphocytes to produce a chemotactic lymphokine. These digested C3 preparations possess only minimal inherent chemotactic properties. The response is completely inhibited by prior adsorption of the trypsin-derived C3 fragments with insolubilized anti-C3. The active C3 fragment is C3b as demonstrated by the elution of activity in the exclusion volume of Sephadex G-75 (m.w. > 50,000 daltons). The C3b-dependent cellular-derived chemotactic factor is present in the supernatants of B cell cultures and totally absent in those of T cell cultures. Generation of this lymphokine occurs in the absence of cellular proliferation.

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