Formation and Activation of a Cyclin E-cdk2 Complex During the G 1 Phase of the Human Cell Cycle
- 18 September 1992
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 257 (5077) , 1689-1694
- https://doi.org/10.1126/science.1388288
Abstract
Human cyclin E, originally identified on the basis of its ability to function as a G1 cyclin in budding yeast, associated with a cell cycle-regulated protein kinase in human cells. The cyclin E-associated kinase activity peaked during G1, before the appearance of cyclin A, and was diminished during exit from the cell cycle after differentiation or serum withdrawal. The major cyclin E-associated kinase in human cells was Cdk2 (cyclin-dependent kinase 2). The abundance of the cyclin E protein and the cyclin E-Cdk2 complex was maximal in G1 cells. These results provide further evidence that in all eukaryotes assembly of a cyclin-Cdk complex is an important step in the biochemical pathway that controls cell proliferation during G1.Keywords
This publication has 17 references indexed in Scilit:
- Cyclin a is required for the onset of DNA replication in mammalian fibroblastsCell, 1991
- A novel cyclin gene from Drosophila complements CLN function in yeast.Genes & Development, 1991
- Cell Cycle Regulation of Histone H1 Kinase Activity Associated with the Adenoviral Protein E1AScience, 1991
- Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene familyCell, 1991
- Isolation of three novel human cyclins by rescue of G1 cyclin (cln) function in yeastCell, 1991
- Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycleCell, 1991
- Human D-type cyclinCell, 1991
- A novel cyclin encoded by a bcl1-linked candidate oncogeneNature, 1991
- Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin BNature, 1990
- A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cellsCell, 1989