TGFβ promotes conversion of CD16+peripheral blood NK cells into CD16−NK cells with similarities to decidual NK cells
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- 27 February 2007
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 104 (9) , 3378-3383
- https://doi.org/10.1073/pnas.0611098104
Abstract
During pregnancy the uterine decidua is populated by large numbers of natural killer (NK) cells with a phenotype CD56superbrightCD16−CD9+KIR+distinct from both subsets of peripheral blood NK cells. Culture of highly purified CD16+CD9−peripheral blood NK cells in medium containing TGFβ1 resulted in a transition to CD16−CD9+NK cells resembling decidual NK cells. Decidual stromal cells, when isolated and culturedin vitro, were found to produce TGFβ1. Incubation of peripheral blood NK cells with conditioned medium from decidual stromal cells mirrored the effects of TGFβ1. Similar changes may occur upon NK cell entry into the decidua or other tissues expressing substantial TGFβ. In addition, Lin−CD34+CD45+hematopoietic stem/progenitor cells could be isolated from decidual tissue. These progenitors also produced NK cells when cultured in conditioned medium from decidual stromal cells supplemented with IL-15 and stem cell factor.Keywords
This publication has 45 references indexed in Scilit:
- Evidence for discrete stages of human natural killer cell differentiation in vivoThe Journal of Experimental Medicine, 2006
- A Human CD34(+) Subset Resides in Lymph Nodes and Differentiates into CD56brightNatural Killer CellsImmunity, 2005
- Dual role for TGF-?1 in apoptosisCytokine & Growth Factor Reviews, 2005
- Human Decidual Natural Killer Cells Are a Unique NK Cell Subset with Immunomodulatory PotentialThe Journal of Experimental Medicine, 2003
- CXCL12 expression by invasive trophoblasts induces the specific migration of CD16– human natural killer cellsBlood, 2003
- What does it take to make a natural killer?Nature Reviews Immunology, 2003
- Expression of killer cell inhibitory receptors on human uterine natural killer cellsEuropean Journal of Immunology, 1997
- Interleukin (IL) 15 is a novel cytokine that activates human natural killer cells via components of the IL-2 receptor.The Journal of Experimental Medicine, 1994
- Expression of a functional c-kit receptor on a subset of natural killer cells.The Journal of Experimental Medicine, 1993
- Expression of Adhesion Molecules by Human Decidual Large Granular LymphocytesCellular Immunology, 1993