Cellular processing of the neurotrophin precursors of NT3 and BDNF by the mammalian proprotein convertases
Open Access
- 5 February 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 379 (3) , 247-250
- https://doi.org/10.1016/0014-5793(95)01520-5
Abstract
In order to define the enzymes responsible for the maturation of the precursors of brain‐derived neurotrophic factor (proBDNF) and neurotrophin‐3 (proNT3), we have analysed their biosynthesis and intracellular processing by the proprotein convertases furin, PC1, PC2, PACE4, PC5 and its isoform PC5/6‐B. In these studies, we utilized a vaccinia virus expression system in either BSC40 or the furin activity‐deficient LoVo cells. Results demonstrated that in both cells furin and, to a lesser extent, PACE4 and PC5/6‐B effectively process proBDNF and proNT3. Furthermore, we have determined that human proNT3 is sulfated, suggesting that processing of proNT3 occurs following the arrival of the precursor to the Trans Golgi Network.Keywords
This publication has 20 references indexed in Scilit:
- Neurotrophic Factors: Two Are Better Than OneScience, 1994
- Functions of the neurotrophins during nervous system development: What the knockouts are teaching usCell, 1994
- The family of subtilisin/kexin like pro-protein and pro-hormone convertases: Divergent or shared functionsBiochimie, 1994
- Identification of an isoform with an extremely large Cys‐rich region of PC6, a Kex2‐like processing endoproteaseFEBS Letters, 1993
- Distribution and regulation of the prohormone convertases PC1 and PC2 in the rat pituitaryMolecular Endocrinology, 1992
- Neurotrophin-5: A novel neurotrophic factor that activates trk and trkBNeuron, 1991
- Detection of brain-derived neurotrophic factor-like activity in fibroblasts and Schwann cells: Inhibition by antibodies to NGFNeuron, 1991
- Neurotrophin-3: a Neurotrophic Factor Related to NGF and BDNFScience, 1990
- The Nerve Growth Factor 35 Years LaterScience, 1987
- Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factorNature, 1983