Relation between Thickness and Interference Colors of Biological Ultrathin Section

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Abstract
Six sections making up a set were chosen according to interference colors, gray to blue, and were piled and mounted on a grid in order of thickness, from the thinnest to the thickest, and re-embedded. Sections were then cut perpendicular to the pile of the original sections. Electron micrographs of the resectioned sections were taken. The layers which can be seen on the micrographs represent the original section thicknesses and their thicknesses were measured on the micrograph. Actual thicknesses of the six sections were as follows: gray 260 to 290 Å, silver 580 to 640 Å, gold 960 to 1, 020 Å, reddish-gold 1,300 to 1, 530 Å, purple 1, 700 to 1, 830 Å, and blue 1, 930 to 2, 050 Å.

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