Expression and Regulation of aERD2, a Gene Encoding the KDEL Receptor Homolog in Plants, and Other Genes Encoding Proteins Involved in ER-Golgi Vesicular Trafficking.
Open Access
- 1 June 1995
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 7 (6) , 667-676
- https://doi.org/10.1105/tpc.7.6.667
Abstract
AERD2 and aSAR1 of Arabidopsis are functional homologs of yeast genes encoding proteins essential for endoplasmic reticulum (ER)-to-Golgi transport. The regulation of these secretory pathway genes in yeast, mammals, and plants is not known. High levels of expression of aERD2 and aSAR1 were observed in roots, flowers, and inflorescence stems, with the highest levels being detected in roots. The aSAR1 transcript levels were highest in young leaves and declined during leaf maturation. Low levels of aERD2 were detected in both young and fully mature leaves when compared with roots. In situ hybridization showed that trichomes accumulate more aERD2 transcript as the leaf expands, whereas aSAR1 is expressed equally in all leaf cell types. Treating plants with tunicamycin, a drug that blocks N-glycosylation in the ER, or with cold shock, known to block secretory protein transport, led to a marked accumulation of aERD2 and aSAR1 transcripts. The Arabidopsis ARF gene, which encodes a GTPase probably involved in Golgi vesicle traffic, was not affected by these treatments. This study is an essential first step toward understanding the regulation of genes that encode proteins involved in vesicular trafficking.Keywords
This publication has 26 references indexed in Scilit:
- Mechanisms of intracellular protein transportNature, 1994
- The Arabidopsis endoplasmic reticulum retention receptor functions in yeast.Proceedings of the National Academy of Sciences, 1993
- Expression and Localization of Plant Protein Disulfide IsomerasePlant Physiology, 1993
- Bean homologs of the mammalian glucose‐regulated proteins: induction by tunicamycin and interaction with newly synthesized seed storage proteins in the endoplasmic reticulumThe Plant Journal, 1992
- Protein disulfide isomerase. A multifunctional protein resident in the lumen of the endoplasmic reticulum.Journal of Biological Chemistry, 1992
- ADP-Ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: A novel role for a GTP-binding proteinCell, 1991
- A human homologue of the yeast HDEL receptorNature, 1990
- ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathwayCell, 1990
- Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathwayCell, 1990
- Messenger RNA for G1 protein of French bean seeds: Cell-free translation and product characterizationProceedings of the National Academy of Sciences, 1978