Molecular cloning and analysis of theCRY1gene: a yeast ribosomal protein gene
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (2) , 403-420
- https://doi.org/10.1093/nar/11.2.403
Abstract
Using cloned DNA from the vicinity of the yeast mating type locus (MAT) as a probe, the wild type allele of the cryptopleurine resistance gene CRY1 has been isolated by the technique of chromosome walking and has been shown to be identical to the gene for ribosomal protein 59. A recessive cryR1 allele has also been cloned, using the integration excision method. The genetic distance from MAT to CRY1 is 2.2 cM, while the physical distance is 21 kb, giving a ratio of about 10 kb/cM for this interval. The phenotypic expression of both plasmid borne alleles of the gene can be detected in vivo. The use of this gene as a hybridization probe to examine RNA processing defects in the rna 2, rna 3, rna 4, rna 8, and rna 11 mutants is also discussed.Keywords
This publication has 38 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Quantitation of the specific interaction of [14a-3H] cryptopleurine with 80S and 40S ribosomal species from the yeast Saccharomyces cerevisiaeBiochemistry, 1982
- Yeast use translational control to compensate for extra copies of a ribosomal protein geneCell, 1982
- The effect of temperature-sensitive RNA mutants on the transcription products from cloned ribosomal protein genes of yeastCell, 1981
- Chromosomal distribution of ribosomal protein genes in the mouseCell, 1981
- DNA rearrangements associated with a transposable element in yeastCell, 1980
- The structure of transposable yeast mating type lociCell, 1980
- Isolation of a circular derivative of yeast chromosome III: implications for the mechanism of mating type interconversionCell, 1979
- Overlap hybridization screening: Isolation and characterization of overlapping DNA fragments surrounding the leu2 gene on yeast chromosome IIIGene, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977