SEPARATION OF ALLELIC VARIANTS BY TWO-DIMENSIONAL ELECTROPHORESIS
- 1 January 1982
- journal article
- research article
- Vol. 34 (2) , 209-215
Abstract
The resolving power of 2-dimensional (2-D) gel electrophoresis was tested using 17 allele products at 5 loci. Standard O''Farrell gels could separate 13 of these isozymes. The addition of a 2nd pH gradient made it possible to separate all but 1 of the variant proteins. 2-D gel electrophoresis can resolve more than 90% of variants originally detected by 1-dimensional (1-D) electrophoresis. The implications of these results for the low estimates of average heterozygosity obtained in surveys using 2-D gel electrophoresis are discussed.This publication has 23 references indexed in Scilit:
- Rate of spontaneous mutation at human loci encoding protein structure.Proceedings of the National Academy of Sciences, 1980
- Analyses of genetic variants of l-glycerol-3-phosphate dehydrogenase in Drosophila melanogaster by two-dimensional gel electrophoresis and immunoelectrophoresisBiochemical Genetics, 1980
- Are abundant proteins less variable?Nature, 1980
- Multiple structural genes for mouse amylaseBiochemical Genetics, 1980
- Genetic heterozygosity in a natural population of Mus musculus assessed using two-dimensional electrophoresisNature, 1980
- Human heterozygosity: a new estimate.Proceedings of the National Academy of Sciences, 1979
- Heterogeneous heterozygosities in Mus musculus populationsProceedings of the Royal Society of London. B. Biological Sciences, 1977
- Polymorphism and the subunit structure of enzymes: a contribution to the neutralist-selectionist controversy.Proceedings of the National Academy of Sciences, 1977
- Albumin Yanomama‐2, a ‘private’ polymorphism of serum albuminAnnals of Human Genetics, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970