Alveolar bubble-stability method in the study of lung alveolar surfactant.

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Abstract
Modifications, standardization, normal values in dogs, and evaluation of the Pattle alveolar bubble-stability method for surfactant estimation are reported. Normal stability ratio (SR) in dogs, using the mean value of 3 groups of 10 bubbles, was .83 with a standard deviation of 0.5 A. A slight but significant decrease in SR was observed in lung samples obtained 2-6 hr. after death and after storage of lung samples for 7-60 days at [long dash]10 C. No significant change was shown after 3 days storage. The sequential type of statistical analysis as may be used in surfactant investigations is presented. Following pulmonary artery ligation the SR in nonatelectatic areas of the ligated lung was significantly lower than that in the nonligated lung, suggesting that the method is sufficiently sensitive to differentiate between gradations of normal and further emphasizing the role of blood flow in synthesis of surfactant. Advantages of the method are enumerated.

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