Entero-hepatischer Kreislauf von C19-Steroiden, II. Rekonjugation von [7-3H]Androstenolon-[35S]sulfat und [7-3H]Androstenolon-[14C]glucuronid in Dünndarm und Leber des Meerschweinchens
Using doubly labelled compounds, the reconjugation reactions of androstenolon sulfate and androstenolone glucuronide in the small intestine and in the liver of guinea pigs was investigated by in vivo experiments. Androstenolone sulfate passed the intestinal wall as well as the liver without hydrolysis. In the intestinal wall one third was converted into steroid sulfatide, which underwent partial hydrolysis in the liver. Androstenolone glucuronide was hydrolyzed in the intestine wall and reconjugated with sulfate; only 1.7% passed the intestinal wall unhydrolyzed. In the liver, the conjugate was secreted into bile without hydrolysis and into the peripheral circulation only after extensive hydrolysis and reconjugation with sulfate.