Origin of Antibody-Dependent, Lymphoid-Cell-Independent Hemolytic Plaques in Rabbit Spleen Cell Cultures

Publisher Website
Google Scholar
Abstract
This report describes a phenomenon that may be a significant source of error in the data acquired from hemolytic plaque assays of lymphocyte cultures. Background antibody in the normal-serum supplement of the culture medium does not actually support initiation of a vigorous primary-type plaque-forming cell response to sheep erythrocytes (SE). Instead, the background antibody causes the development of numerous spurious lymphoid-cell-independent plaques. Primary-type rabbit spleen cell responses in vitro were low in culture media supplemented with fetal bovine serum, a serum which does not contain background antibodies to SE.

This publication has 0 references indexed in Scilit: