ACCURACY OF HDL-CHOLESTEROL AND HDL-APOLIPOPROTEIN-A-I DETERMINATIONS AFTER PHOSPHOTUNGSTATE-MGCL2-PRECIPITATION OF APOLIPOPROTEIN-B-CONTAINING LIPOPROTEINS

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 19  (5) , 273-278
Abstract
With optimal concentrations of reagents a complete precipitation of apolipoprotein B-containing lipoproteins and only a small coprecipitation of HDL can be achieved using the phosphotungstate/MgCl2 precipitation method. The accuracy of the precipitation is not influenced by triglyceride concentrations < 4.0 mmol/l. Similarly, in most hypertriglyceridemic sera (> 4.0 mmol/l) a complete precipitation of apolipoprotein B-containing lipoproteins and a complete recovery of HDL can be observed. In addition to the HDL [high density lipoprotein] cholesterol determination, apolipoprotein A-I was determined by kinetic nephelometry. In hypertriglyceridemic sera (> 4.56 mmol/l) precipitation of apolipoprotein B-containing lipoproteins must be carried out prior to the nephelometric determination of apolipoprotein A-I.