Somatic Embryogenesis in Tissue Cultures of Pecan
Open Access
- 1 February 1987
- journal article
- Published by American Society for Horticultural Science in HortScience
- Vol. 22 (1) , 128-130
- https://doi.org/10.21273/hortsci.22.1.128
Abstract
Tissue cultures of pecan [Carya illinoensis (Wangenh.) C. Koch] were initiated from embryo explants collected weekly from 12 weeks post-pollination until fruit maturity. Three cultures derived from immature embryos collected 14 weeks postpollination produced primary somatic embryos within 1 month following transfer from modified woody plant medium (WPM) with 2.0 mgliter−1 2,4-D and 0.25 mg liter−1 BA in the light to hormone-free medium in the dark. Scanning electron microscopy documented the development of secondary embryos, which followed a globular, heartshaped, and torpedo-stage developmental sequence. Chemical names used: (2,4-di-chlorophenoxy) acetic acid (2,4-D), N-(phenylmethyl)-1H-purin-6-amine (BA), and 1H-indole-3-butanoic acid (IBA).Keywords
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