Low specificity of anti‐tissue transglutaminase antibodies in patients with primary biliary cirrhosis
- 1 January 2006
- journal article
- research article
- Published by Wiley in Journal of Clinical Laboratory Analysis
- Vol. 20 (5) , 184-189
- https://doi.org/10.1002/jcla.20130
Abstract
The association between celiac disease (CD) and primary biliary cirrhosis (PBC) is well documented in medical literature; however, a high frequency of false positive results of the anti‐transglutaminase (anti‐tTG) test has been reported in patients with PBC. To verify if the positive results for anti‐tTG autoantibody are false positives due to cross reactivity with mitochondrial antigens, we studied 105 adult patients affected with PBC, positive for anti‐mitochondrial M2 antibodies. Anti‐tTG IgA antibodies were studied by using six different immunoenzymatic assays that employ the tTG antigen obtained from different sources (human recombinant, placenta, red blood cells, and guinea pig liver). On the whole, 28 out of 105 PBC subjects tested positive for anti‐tTG IgA antibodies, but only two were eventually found to be affected by CD; the other 26 were shown to be false positive. The specificity of the various antigenic substrates ranged from 88.5% of the human erythrocytes tTG to 97.1% of the human recombinant tTG. The results of this study showed that a true association between PBC and CD was present in only 2% of the patients and that, in most cases, the false positive results were attributable to the type of substrate utilized in the assay. J. Clin. Lab. Anal. 20:184–189, 2006.Keywords
This publication has 51 references indexed in Scilit:
- Prevalence and Clinical Significance of Immunoglobulin A Antibodies against Tissue Transglutaminase in Patients with Diverse Chronic Liver DiseasesClinical and Vaccine Immunology, 2005
- False positive reactions for IgA and IgG anti-tissue transglutaminase antibodies in liver cirrhosis are common and method-dependentClinica Chimica Acta; International Journal of Clinical Chemistry, 2005
- Characterization of recombinant monoclonal IgA anti–PDC-E2 autoantibodies derived from patients with PBCHepatology, 2002
- Characterization of recombinant monoclonal IgA anti—PDC-E2 autoantibodies derived from patients with PBCHepatology, 2002
- Mucosal Immunity and Primary Biliary Cirrhosis: Presence of Antimitochondrial Antibodies in UrineHepatology, 2000
- Penetration and Co-localization in MDCK Cell Mitochondria of IgA Derived from Patients with Primary Biliary CirrhosisJournal of Autoimmunity, 1998
- High prevalence of undiagnosed coeliae disease in 5280 Italian students screened by antigliadin antibodiesActa Paediatrica, 1995
- Molecular mimicry in primary biliary cirrhosis. Evidence for biliary epithelial expression of a molecule cross-reactive with pyruvate dehydrogenase complex-E2.Journal of Clinical Investigation, 1993
- The predominance of IgG3 and IgM isotype antimitochondrial autoantibodies against recombinant fused mitochondrial polypeptide in patients with primary biliary cirrhosisHepatology, 1988
- Incidence and Prevalence of Primary Biliary Cirrhosis in a Denned Population in SwedenScandinavian Journal of Gastroenterology, 1985