DETERMINATION OF CLONALITY IN ACUTE NONLYMPHOCYTIC LEUKEMIA BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM AND METHYLATION ANALYSIS
- 1 March 1987
- journal article
- research article
- Vol. 1 (3) , 226-230
Abstract
Determination of cellular clonality in hematological malignancies provides fundamental information that is important in understanding the pathogenesis of these disorders. We present here an extension of one approach to accomplish this that is based on the interpretation of different methylation patterns on active and inactive X chromosomes within the region of the hypoxanthine-guanine phosphoribosyltransferase gene spanned by a restriction fragment length polymorphism. The successful application of the method to determine clonality is described for three female patients with acute nonlymphocytic leukemia.This publication has 21 references indexed in Scilit:
- Philadelphia chromosomal breakpoints are clustered within a limited region, bcr, on chromosome 22Cell, 1984
- Immunoglobulin-Gene Rearrangements as Unique Clonal Markers in Human Lymphoid NeoplasmsNew England Journal of Medicine, 1983
- Multiclonal Origin of Polyps in Gardner SyndromeScience, 1983
- A three-allele restriction-fragment-length polymorphism at the hypoxanthine phosphoribosyltransferase locus in man.Proceedings of the National Academy of Sciences, 1983
- ACUTE NONLYMPHOCYTIC LEUKEMIA - HETEROGENEITY OF STEM-CELL ORIGIN1981
- Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.Proceedings of the National Academy of Sciences, 1979
- Multicellular Origin of Parathyroid “Adenomas”New England Journal of Medicine, 1977
- Clonal origin of human tumorsBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1976
- The Clonal Evolution of Tumor Cell PopulationsScience, 1976
- ELECTROPHORETIC HETEROGENEITY OF GLUCOSE-6-PHOSPHATE DEHYDROGEN ASE AND ITS RELATIONSHIP TO ENZYME DEFICIENCY IN MANProceedings of the National Academy of Sciences, 1962