AN APPRAISAL OF METHODS OF TISSUE CHLORIDE ANALYSIS: THE TOTAL CARCASS CHLORIDE, EXCHANGEABLE CHLORIDE, POTASSIUM AND WATER OF THE RAT 1

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Abstract
Three methods of tissue chloride analysis were compared (1) The open Carius method with tissue digestion by boiling on a hot plate; (2) The open Carius method with digestion in a boiling water bath; (3) An alkaline ash procedure with determination of chloride by the microdiffusion technique of Conway. The 3d method gave better agreement with respect to repeated analyses and more accurate recovery of chloride when added to tissue. Although the 2d method yielded results that were less reproducible than those of the 3d method the averages of replicate determinations by the 2 methods were in good agreement. The 1st method gave results that were significantly low. The exchangeable chloride was measured in 45 rats by determining the volume of distribution of bromide. The same rats were then subjected to carcass analysis by methods described. The total carcass chloride in rats by methods 2 and 3 closely approximated the exchangeable chloride. Substituting isotonic saline for drinking water did not alter the exchangeable chloride or carcass chloride. Total body chloride, water and K exhibit a linear relationship when plotted against lean body mass or fat free dried solid.