Immunogenicity of Toxic and Detoxified Endotoxin Preparations

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Abstract
Several-times repeated injections of heat-killed Serratia marcescens cells, toxic endotoxins, or CH3OKdetoxifiedderivativethereof (endotoxoid-2) into rabbits result in a comparable antibody titer after 8-10 injections. The antibody titer produced by toxic preparations shows a rapid initial increase, while endotoxoid-2 produces a lower titer of circulating antibodies in the 1st 10 days. While toxic preparations produce both 2-mer-captoethanol resistant and sensitive antibodies from the beginning of the immunization, endotoxoid-2 produces almost exclusively 2-mercap-toethanol sensitive antibodies in the 1st 10 days. Simultaneous with the appearance of 2-mercaptoethanol resistant antibodies, the level of total antibodies rises rapidly. A single injection of toxic endotoxin produces a rapid antibody production which starts to decline after approximately 2 weeks. Booster injections bring this antibody level back to previous highs, but hardly any additional effect can be observed. A single injection of endotoxoid-2 gives a slow output of antibodies, which seems to remain stable for 1 month. Booster injection of endotoxoid-2 results in a strong increase of antibody production.

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