Binding of cardiotoxin analogue III from Formosan cobra venom to FL cells

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Abstract
The binding equilibrium at 37 or 0°C of 125I‐cardiotoxin analogue III (CT III) to fetal lung (FL) cells (cultured human amnion cells) was achieved within 1 h, and the binding at 37°C was irreversible. The Scatchard analysis at 37°C on the binding of 125I‐CT III indicated that FL cells had two types of binding sites with different association constants. The association constant and the number of high‐affinity sites was 1.1 × 1010 mol−1 or 2.8 × 106 per FL cell, respectively. At 37 or 0°C, the cytotoxicity of CT III paralleled the amount of bound CT III to FL cells, and at 37°C was inhibited by the presence of acidic phospholipids.