Fluorescent Amplifying Recognition for DNA G-Quadruplex Folding with a Cationic Conjugated Polymer: A Platform for Homogeneous Potassium Detection
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- 12 August 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 127 (35) , 12343-12346
- https://doi.org/10.1021/ja051507i
Abstract
Single-stranded DNA with G-rich sequences can fold into secondary structures, G-quadruplexes, via intramolecular hydrogen-bonding interactions. This conformational change can be detected by a homogeneous assay method based on fluorescence resonance energy transfer (FRET) from a water-soluble cationic conjugated polymer (CCP) to a fluorescein chromophore labeled at the terminus of the G-quadruplex DNA. The space charge density around the DNA controls the efficiency of FRET from the CCP to the fluorescein. The higher FRET efficiency for the CCP/G-quadruplex pair is correlated to the stronger electrostatic interactions between the more condensed G-quadruplex and the CCP in comparison to the CCP/ssDNA pair. Since the potassium ion can specifically bind to the G-quadruplex DNA, the G-quartet-DNA/CCPs assembly can also be used as a platform to sense the potassium ion in water with high selectivity and sensitivity.Keywords
This publication has 16 references indexed in Scilit:
- Fluorescent Polymeric Transducer for the Rapid, Simple, and Specific Detection of Nucleic Acids at the Zeptomole LevelJournal of the American Chemical Society, 2004
- KSb5S8: A Wide Bandgap Phase‐Change Material for Ultra High Density Rewritable Information StorageAdvanced Materials, 2003
- DNA duplex–quadruplex exchange as the basis for a nanomolecular machineProceedings of the National Academy of Sciences, 2003
- A Novel Potassium Sensing in Aqueous Media with a Synthetic Oligonucleotide Derivative. Fluorescence Resonance Energy Transfer Associated with Guanine Quartet−Potassium Ion Complex FormationJournal of the American Chemical Society, 2002
- Energy Transfer in Mixtures of Water- Soluble Oligomers: Effect of Charge, Aggregation, and Surfactant ComplexationAdvanced Materials, 2002
- A New Model for the K+-Induced Macromolecular Structure of Guanosine 5‘-Monophosphate in SolutionBiochemistry, 1999
- Multistranded DNA structuresCurrent Opinion in Structural Biology, 1999
- Benzo-15-crown-5 Fluoroionophore/γ-Cyclodextrin Complex with Remarkably High Potassium Ion Sensitivity and Selectivity in WaterJournal of the American Chemical Society, 1999
- Guanine quartetsCurrent Opinion in Structural Biology, 1993
- Telomeric DNA dimerizes by formation of guanine tetrads between hairpin loopsNature, 1989