Ultrastructural localization of IgA and IgG receptors on oocysts, sporocysts, sporozoites, and merozoites of Eimeria falciformis

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Abstract
The localization of parasite-specific IgA and IgG immunoglobulins on Eimeria falciformis oocysts, sporocysts, sporozoites, and merozoites was examined by immunoelectron microscopy. Parasites were fixed in glutaraldehyde, incubated with heat-inactivated sera or gut contents from normal or specifically immunized mice, reacted with ferritin-conjugated or colloidal gold-conjugated sheep or goat antimouse IgA or IgA antibody and prepared for transmission electron microscopy. Other purified samples of sporozoites or merozoites were exposed to sera or gut contents, fixed in 0.15% glutaraldehyde, and then incubated with ferritin-conjugated or colloidal gold-conjugated sheep or goat antimouse antibody. Parasite-specific IgA and IgG receptors were detected on the plasmalemma of sporozoites and merozoites. Specific IgG receptors were also present on the inner and outer layers of the oocyt wall, and on the inner surface of the sporocyst wall. Live sporozoites and merozoites shed immune complexes at their posterior ends. No internal alternations were detected ultrastructurally in sporozoites or merozoites treated with parasite-specific IgA or IgG antibodies.

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