Mapmodulin: A possible modulator of the interaction of microtubule-associated proteins with microtubules
Open Access
- 13 May 1997
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 94 (10) , 5084-5089
- https://doi.org/10.1073/pnas.94.10.5084
Abstract
We have purified and characterized a 31-kDa protein named mapmodulin that binds to the microtubule-associated proteins (MAPs) MAP2, MAP4, and tau. Mapmodulin binds free MAPs in strong preference to microtubule-associated MAPs, and appears to do so via the MAP’s tubulin-binding domain. Mapmodulin inhibits the initial rate of MAP2 binding to microtubules, a property that may allow mapmodulin to displace MAPs from the path of organelles translocating along microtubules. In support of this possibility, mapmodulin stimulates the microtubule- and dynein-dependent localization of Golgi complexes in semi-intact CHO cells. To our knowledge, mapmodulin represents the first example of a protein that can bind and potentially regulate multiple MAP proteins.Keywords
This publication has 33 references indexed in Scilit:
- Microtubule organization and dynamics dependent on microtubule-associated proteinsPublished by Elsevier ,2004
- Removal of MAP4 from microtubules in vivo produces no observable phenotype at the cellular level.The Journal of cell biology, 1996
- Molecular Identification of I1PP2A, a Novel Potent Heat-Stable Inhibitor Protein of Protein Phosphatase 2ABiochemistry, 1996
- Interactions of Microtubule-Associated Protein MAP2 with Unpolymerized and Polymerized Tubulin and Actin Using a 96-Well Microtiter Plate Solid-Phase ImmunoassayBiochemistry, 1994
- Steric inhibition of cytoplasmic dynein and kinesin motility by MAP2Cell Motility, 1993
- Microtubule stabilization by assembly‐promoting microtubule‐associated proteins: A repeat performanceCell Motility, 1992
- Beyond self-assembly: From microtubules to morphogenesisCell, 1986
- Analysis of the microtubule-binding domain of MAP-2.The Journal of cell biology, 1985
- Reconstitution of the transport of protein between successive compartments of the golgi measured by the coupled incorporation of N-acetylglucosamineCell, 1984
- Electron microscopy of map 2 (microtubule-associated protein 2)Journal of Ultrastructure Research, 1982