Geometry of the phosphodiester backbone in the A form of deoxyribonucleic acid determined by phosphorus-31 nuclear magnetic resonance spectroscopy
- 17 February 1981
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 20 (4) , 745-750
- https://doi.org/10.1021/bi00507a013
Abstract
31P NMR spectra were obtained from poly(dA-dT) .cntdot. poly(dA-dT) fibers which gave an X-ray diffraction pattern similar to that of the A form of natural deoxyribonucleic acid (DNA). The A form of poly(dA-dT) .cntdot. poly(dA-dT) has a single uniform backbone conformation; the orientation of the phosphodiester group relative to the helical axis was .beta. = 70.degree. and .gamma. = 52.degree.. The 31P NMR spectra of poly(dA-dT) .cntdot. poly(dA-dT) contrasted with the 31P NMR spectra of the A form of natural DNA, which exhibited an unusual line shape. The origins of the abnormalities in the line shape for the A form of natural DNA are discussed in terms of phosphodiester orientations.This publication has 7 references indexed in Scilit:
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