Biological and Immunological Activity of a Thyrocalcitonin Produced by the Solid-Phase Method of Peptide Synthesis1

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Abstract
We have used a modified solidphase method! of peptide synthesis to prepare a thyrocalcitonin closely resembling the pure human hormone in its chemical and chromatographic properties. The partially purified solid phase product had a specific biological activity of approximately 25 MRC U/mg of peptide when assayed by the sc or iv route in young rats against pure human MRC standard thyrocalcitonin (assigned potency, 100 MRC U/mg). The solid—phase product was immunologically equivalent to pure synthetic human standard thyrocalcitonin when the two were compared using two different antisera and using either synthetic standard hormone or the solid—phase product as the radiolabeled antigen. One antiserum had been elicited by immunizing guinea pigs with the solid—phase peptide and the other antiserum by immunizing with the standard. Solid—phase synthesis may offer a convenient means of obtaining calcitonins of specified amino acid sequence for immunological and biological studies.(Endocrinology92: 1780, 1973)

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