The Isolation, Propagation, and Activity of a Viral Agent Associated With Dentinal Destruction

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Abstract
Dentinal decay from teeth in the mouth or freshly extracted teeth was ground with or without preheated washed sand in an autoclaved dental mortar and pestle. The material was brought to a pasty consistency with physiological saline and passed through a Seitz filter (0.2-0.3[mu]). 0.5 ml. of the filtrate was injd. into the chorio-allantoic membrane of chick embryos and the opening in the egg was sealed with dental wax. The eggs were opened after intervals of 24-120 hrs., 48 hrs. being the preferred incubation period. Several serial passages with fresh starting material were carried for up to 25 passages. Impression slides were made from the chorio-allantoic membrane. Cells of the membrane were stained with Seller''s stain. Inclusion bodies were observed routinely as early as the 7th passage, sometimes earlier.

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