Immunoreactive Trypsins in Sera from Dogs Before and After Induction of Experimental Pancreatitis

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Abstract
Radioimmunoassays for anionic and cationic dog trypsins are described. Characterization of the immunoreactivities in sera from fasting dogs demonstrated the presence of the 2 proenzymes only. Fasting sera from 10 dogs contained anionic and cationic trypsinogen in concentrations between 17-110 .mu.g/l and 7-19 .mu.g/l, respectively. Induction of experimental pancreatitis in dogs was accompanied by a large increase of immunoreactive anionic and cationic trypsins in the circulation. During the progress of the pancreatitis, immunoreactive trypsin with larger MW than trypsinogen appeared. This high MW immunoreactive trypsin was not seen in serum after i.v. injection of pancreatic juice in dogs. The high MW immunoreactive trypsin probably consists of trypsin in complex with protease inhibitors. In vitro studies showed that the immunoreactivity of trypsin decreased considerably after binding to .alpha.1-antitrypsin or .alpha.-macroglobulins.