A quantitative study of the influence of fixation on immunoperoxidase staining of rectal mucosal plasma cells.
- 1 April 1980
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 33 (4) , 361-364
- https://doi.org/10.1136/jcp.33.4.361
Abstract
A quantitative morphometric study of immunoglobulin-containing plasma cells in rectal biopsies from 9 patients with ulcerative colitis and 3 patients with the irritable colon syndrome was presented. Fixation of the biopsy specimens with formol-sublimate, a mercuric fixative, resulted in better staining with the immunoperoxidase method and, by comparison with formol saline, higher cell counts were always obtained.Keywords
This publication has 9 references indexed in Scilit:
- TISSUE EOSINOPHILS IN ULCERATIVE-COLITIS1979
- The influence of fixation on immunoperoxidase staining of plasmacells in paraffin sections of intestinal biopsy specimensHistochemistry and Cell Biology, 1977
- Transport of Specimens for Clinical Chemistry AnalysisAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1977
- Morphological methods in the study of the gut immune system in man.Journal of Clinical Pathology, 1976
- Immunohistochemical studies of the formation and epithelial transport of immunoglobulins in normal and diseased human intestinal mucosa.1976
- The plasma cells in inflammatory disease of the colon: A quantitative studyJournal of Clinical Pathology, 1974
- The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibodyJournal of Clinical Pathology, 1974
- Renal Function Tests Suitable for Clinical PracticeAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1972
- Bilirubin Standards and the Determination of Bilirubin by Manual and Technicon AutoAnalyzer MethodsAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 1971