SEQUENTIAL ALKALINE-PHOSPHATASE CHANGES IN HYPERPLASTIC AND NEOPLASTIC UROTHELIUM OF MICE FED 2-ACETYLAMINOFLUORENE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 1  (3) , 223-231
Abstract
The urinary bladder was studied in 430 BALB/c mice fed a stock diet containing 0, 100, or 500 ppm 2-acetylaminofluorene [2-AAF]. At 12 wk, nearly all mice on the high [500 ppm] 2-AAF diet showed bladder epithelial hyperplasia with epithelial decrease and stromal increase in alkaline phosphatase. There were frequently many cytoplasmic vacuoles, with eosinophilic inclusions often staining like alkaline phosphatase, in the superficial polyploid and upper intermediate epithelial cell layers. After withdrawing 2-AAF at 12 wk, such changes decreased, but 1 of 4 females and 2 of 4 males sacrificed at 51 wk had papillary transitional cell carcinomas. Mice on 100 ppm 2-AAF developed similar changes more slowly. At 57 wk, epithelial changes were moderate or were marked in nearly all males and decreased only slightly after withdrawal of 2-AAF at 54-57 wk. In females, cytoplasmic vacuolation and other changes were milder and virtually disappeared at 67 wk, 13 wk after withdrawal of 2-AAF. At 57-103 wk, no females on the low 2-AAF diet developed carcinomas, whereas nearly all males, even after withdrawing 2-AAF at 54-57 wk, developed papillary carcinomas. There appeared to be some correlation between the severity and persistence of cytologic damage, evidenced by vacuoles with inclusions, in a given treatment group and the later development of carcinomas in that group.

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