The Monoclonal Antibody W7C5 Defines a Novel Surface Antigen on Hematopoietic Stem Cells
- 1 June 2001
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 938 (1) , 175-183
- https://doi.org/10.1111/j.1749-6632.2001.tb03587.x
Abstract
Abstract: We recently raised a monoclonal antibody, termed W7C5, against a surface antigen that is expressed at low levels on bone marrow and peripheral blood CD34+ stem/progenitor cells but at high levels on fetal liver CD34+ cells. A reasonable staining intensity was achieved using magnetofluorescent liposome conjugates to analyze expression of W7C5 antigen on CD34+CD38− bone marrow (BM) cells. Flow cytometric analyses revealed that W7C5 detects about 50% of immature CD34+CD38− BM cells that coexpressed the differentiation antigens CD164, CD133, and CD172a (SIRPα). In addition, W7C5 also recognized a CD34− BM fraction. These cells were negative for CD117 and CD133, but expressed CD45 and moderate levels of CD164. Injection of selected CD34+W7C5+ and CD34−W7C5+ cells into 55–60‐day‐old fetal sheep resulted in an engraftment of both fractions. Partial amino acid sequence analysis of affinity‐purified lysates of KU‐812 cells revealed that W7C5 detects a novel membrane protein. Together, W7C5 defines a novel molecule that is expressed on CD34+ as well as on CD34− stem cell subsets.Keywords
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