CMP‐N‐acetylneuraminic acid hydroxylase: the first cytosolic Rieske iron‐sulphur protein to be described in Eukarya
- 6 May 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 385 (3) , 197-200
- https://doi.org/10.1016/0014-5793(96)00384-5
Abstract
Electron paramagnetic resonance (EPR) spectroscopy and analysis of the primary structure of the CMP-N-acetylneuraminic acid hydroxylase revealed that this enzyme is the first iron-sulphur protein of the Rieske type to be found in the cytosol of Eukarya. The dithionite-reduced hydroxylase exhibited an EPR signal known to be characteristic for a Rieske iron-sulphur centre (2Fe-2S), the g-values being 1.78, 1.91 and 2.01, respectively. An analysis of the primary structure of the hydroxylase led to the identification of an amino acid sequence, known to be characteristic for Rieske proteins. Furthermore, possible binding sites for cytochrome b 5, the substrate CMP-Neu5Ac and a mononuclear iron centre were also identified.Keywords
This publication has 30 references indexed in Scilit:
- Molecular Cloning of Cytidine Monophospho-N-acetylneuraminic Acid Hydroxylase. REGULATION OF SPECIES- AND TISSUE-SPECIFIC EXPRESSION OF N-GLYCOLYLNEURAMINIC ACIDJournal of Biological Chemistry, 1995
- The nucleotide sequence of the Tn5271 3-chlorobenzoate 3,4-dioxygenase genes (cbaAB) unites the class IA oxygenases in a single lineageMicrobiology, 1995
- The Sialyltransferase “Sialylmotif” Participates in Binding the Donor Substrate CMP-NeuAcJournal of Biological Chemistry, 1995
- Purification and characterization of CMP-N-acetylneuraminic acid hydroxylase from pig submandibular glandsGlycobiology, 1994
- CMP‐N‐acetylneuraminic acid hydroxylase from mouse liver and pig submandibular glandsEuropean Journal of Biochemistry, 1994
- Mouse liver cytidine‐5′‐monophosphate‐N‐acetylneuraminic acid hydroxylaseEuropean Journal of Biochemistry, 1992
- Potential ligands to the [2Fe-2S] Rieske cluster of the cytochrome bc1 complex of Rhodobacter capsulatus probed by site-directed mutagenesisBiochemistry, 1992
- The Nucleotide Sequence of the Equine Herpesvirus 4 Thymidine Kinase GeneJournal of General Virology, 1990
- The Biosynthesis of N-Glycoloylneuraminic Acid Occurs by Hydroxylation of the CMP-Glycoside of N-Acetylneuraminic AcidBiological Chemistry Hoppe-Seyler, 1988
- Mössbauer Studies on the Active Fe …[2Fe‐2S] Site of Putidamonooxin, Its Electron Transport and Dioxygen Activation MechanismEuropean Journal of Biochemistry, 1981