A New Extragenic Suppressor of cya Mutation
- 1 June 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 83 (6) , 1615-1623
- https://doi.org/10.1093/oxfordjournals.jbchem.a132073
Abstract
A strain bearing an extragenic suppressor of cya mutation was isolated as a second-site revertant of an adenylate cyclase deficient strain. The mutant was unable to synthesize cAMP but showed normal fermentation profiles and growth properties on a variety of carbon sources. The site of reversion was mapped in, or near, the structural gene for the cAMP receptor protein. Structural alteration of the protein was directly demonstrated by the following biochemical observations: (i) A 10-fold decrease in the dissociation constant for cAMP, (ii) an acidic shift in the isoelectric point, and (iii) the altered binding properties to λh80d/ac p2 DNA.This publication has 4 references indexed in Scilit:
- A possible involvement of cya gene in the synthesis of cyclic guanosine 3′:5′-monophosphate in E. coliCell, 1977
- Catabolite modulator factor: a possible mediator of catabolite repression in bacteria.Proceedings of the National Academy of Sciences, 1976
- Transducing fragments in generalized transduction by phage P1Journal of Molecular Biology, 1965
- The genetic control and cytoplasmic expression of “Inducibility” in the synthesis of β-galactosidase by E. coliJournal of Molecular Biology, 1959