High-performance microfabricated capillary array electrophoresis systems for DNA analysis

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Abstract
The microfabrication of DNA sample preparation, electrophoretic separation and detection devises is making possible a new generation of high-speed, high-throughput DNA analysis systems. HIgh quality DNA fragment sizing and short tandem repeat analyses have been preformed on microfabricated capillary electrophoresis devices that are 10-100 times faster than conventional electrophoretic separations. Microfabricated capillary array electrophoreses (CAE) plates increase the throughput of these devices by permitting the analysis of multiple samples in parallel. The practical utility of these devices has been improved by developing elastomer sample well and electrode arrays with standard microtiter dish spacings. We initially developed a CAE microplate that can analyze 48 samples using an array of 12 capillaries. More recently, we have developed an improved microplate with an array of 48 capillaries capable of analyzing up to 96 DNA samples in < 8 minutes. These highly parallel, miniaturized DNA analysis systems are a significant step toward fast and inexpensive genetic analysis.

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