CELL-PROLIFERATION IN NORMAL AND ATHEROSCLEROTIC HUMAN AORTA .1. FLOW CYTOFLUOROMETRIC DETERMINATION OF CELLULAR DEOXYRIBONUCLEIC-ACID CONTENT

  • 1 January 1983
    • journal article
    • research article
    • Vol. 48  (4) , 395-398
Abstract
By enzymatic dispersion cells were isolated from intimal and medial layers of healthy and atherosclerotic human aorta. The analysis of cell distribution according to DNA content was made using flow cytofluorometry. Apparently, intimal and medial cell populations are heterogenous with respect to DNA content, and include 2 n and 4 n DNA populations. The 4 n cell content may amount to 19% of cell population. As a rule, the percentage of 4 n cells in intima is lower than in media. In atherosclerotic lesions their content is lower than in uninvolved areas. The 4 n-DNA population includes mono- and binuclear cells.

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