INDIRECT IMMUNOFLUORESCENCE DETECTION OF HUMAN IgM AND IgG ANTIBODIES AGAINST HERPES SIMPLEX VIRUS TYPE 1 INDUCED CELL SURFACE ANTIGENS

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Abstract
An indirect immunofluorescence method, based on the use of infected [human cervical carcinoma] Hela cell coverslip cultures, was developed to demonstrate human Ig[immunoglobulin]M and IgG antibodies against herpes simplex virus (HSV) type 1 induced cell surface antigens. A total of 35 specimens from 20 patients were tested, including patients with a clinical diagnosis of HSV type 1 or type 2 primary infection, patients with recurrent HSV infections, patients without any HSV infections and patients with varicella-zoster virus (VZV) infections. In all patients with primary HSV infection both IgM and IgG antibody response was observed, while patients with recurrent HSV infections showed only IgG antibodies. The direct serological typing of HSV infections was not possible because of cross-reacting antibodies in the IgG and IgM tests. No cross-reactivity was found in this test with HSV and VZV antibodies. The HSV fluorescent IgG and IgM antibody titers paralleled the highly sensitive HSV radioimmunoassay antibody titers very closely, though at a markedly lower level.