Cosmid cloning and transposon mutagenesis in Salmonella typhimurium using phage λ vehicles
- 1 February 1981
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 181 (2) , 153-157
- https://doi.org/10.1007/bf00268420
Abstract
We have constructed a strain of Salmonella typhimurium which contains the malB region from Escherichia coli and carries the bacteriophage λ receptor protein in its outer membrane. Phage λ adsorbs to this strain but cannot grow, thus providing a very useful system for transposon mutagenesis of S. typhimurium using λ vehicles carrying transposons. This system can also be used for cosmid cloning.Keywords
This publication has 40 references indexed in Scilit:
- Is elements and transposonsPlasmid, 1980
- Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10Journal of Molecular Biology, 1979
- Plasmids useable as gene-cloning vectors in an in vitro packaging by coliphage λ: “cosmids”Gene, 1978
- Genetic engineering in Vivo using translocatable drug-resistance elementsJournal of Molecular Biology, 1977
- Translocatable elements in procaryotesCell, 1977
- Maltose Transport in Escherichia coli K12. A Comparison of Transport Kinetics in Wild-Type and lamba-Resistant Mutants with the Dissociation Constants of the Maltose-Binding Protein as Measured by Fluorescence QuenchingEuropean Journal of Biochemistry, 1976
- DNA restriction and modification systems in SalmonellaMolecular Genetics and Genomics, 1974
- Genetic characterization of a bacterial locus involved in the activity of the N function of phage λVirology, 1974
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970