TISSUE-CULTURE ISOLATION AND PRESERVATION OF HUMAN CADAVERIC PANCREATIC-ISLETS

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 81  (3) , 270-273
Abstract
Specimens (6) of human cadaveric pancreas were collagenase-dispersed and placed in tissue culture. Media were changed at 2 day intervals and assayed for insulin and amylase. Experiments were terminated after 8 days, and phase-contrast and light microscopy were performed on cultured tissue. Insulin content in media remained high for 6 days in 5 cases and for 8 days in 4 experiments; this correlated directly with morphologic viability of cultured tissue. Media amylase fell to 0 after 4 days in 4 cases and after 6 days in 2 cases. Tissue culture may be an efficient method for islet cell purification, since acinar tissue and amylase activity disappear, and also for islet preservation.

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