To isolate the tomato Cf-2 resistance gene by map-based cloning, plants recombinant for RFLP markers close to Cf-2 were selected by exploiting the flanking morphological markers yv (yellow virescent) and tl (thiaminless). Using these recombinants, a high-resolution linkage map of the region encompassing the Cf-2 gene has been generated containing several new RFLP markers. Mapping of two YAC clones carrying Lycopersicon esculentum and L. peruvianum DNA, indicates that in both genotypes the physical distance between the two closet flanking markers is less than 40 kb. This study also positions Cf-2 relative to the Mi gene, which confers resistance to root-knot nematodes.