The isolation and sequencing of human gastric inhibitory peptide (GIP)

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Abstract
Human GIP 1–42 and fragments of human GIP corresponding to GIP 10–42, GIP 11–42, and GIP 17–42 were isolated from acid-ethanol extracts of human small intestines with the aid of an anti-GIP serum specific for the extreme C-terminal portion of the GIP molecule. The full sequence of human GIP has been established by Edman degradation of these peptides and fragments thereof by automatic gas-phase sequencing. Human GIP differs from porcine GIP at residues 18 and 34. The sequence of human GIP is thus: Tyr-Ala-Glu-Gly-Thr-Phe-Ile-Ser-Asp-Tyr-Ser-Ile-Ala-Met-Asp-Lys-Ile-His-Gln-Gln-Asp-Phe-5 10 15 20 Val-Asn-Trp-Leu-Leu-Ala-Glu-Lys-Gly-Lys-Lys-Asn-Asp-Trp,Lys-His-Asn-Ile-Thr-Gln. Amino acid 25 30 35 40 residues 18 and 34 are Arg and Ser, respectively, in porcine GIP.