Use of Eukaryotic Native Small Ribosomal Subunits for the Translation of Globin Messenger RNA
- 1 September 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (9) , 3435-3439
- https://doi.org/10.1073/pnas.71.9.3435
Abstract
A highly active in vitro system for the translation of globin mRNA, resulting in more than 10 rounds of translation, is described. The reconstituted system consists of native small ribosomal subunits of rabbit reticulocytes (as a source of initiation factors as well as small ribosomal subunits), large subunits derived from rat liver polysomes by the puromycin-KCl procedure, and a pH 5 fraction obtained from a Krebs ascites cell high speed supernatant. In this system no differences were found between globin messenger ribonucleoprotein and globin mRNA.Keywords
This publication has 17 references indexed in Scilit:
- Initiation of protein synthesis: Evidence for messenger RNA-independent binding of methionyl-transfer RNA to the 40 S ribosomal subunitJournal of Molecular Biology, 1973
- Initiation of mammalian protein synthesis: the importance of ribosome and initiation factor quality for the efficiency of in vitro systemsJournal of Molecular Biology, 1973
- A complex between Met-tRNAF and native 4OS subunits in reticulocyte lysates and its disappearance during incubation with double-stranded RNABiochemical and Biophysical Research Communications, 1972
- Control of globin synthesis: The role of hemeJournal of Molecular Biology, 1972
- Protein tightly bound to globin mRNABiochemical and Biophysical Research Communications, 1972
- Structure and function of mammalian ribosomes: I. Isolation and characterization of active liver ribosomal subunitsJournal of Molecular Biology, 1970
- The Ribosome Cycle in a Reconstituted Cell-Free System from ReticulocytesCold Spring Harbor Symposia on Quantitative Biology, 1969
- The role of the native subribosomal particles of Escherichia coli in polypeptide chain initiation.Proceedings of the National Academy of Sciences, 1967
- Initiation of haemoglobin polypeptide chainsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- Changes in the ribosome distribution during incubation of rabbit reticulocytes in vitroBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1965