Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein
Open Access
- 1 September 1983
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 305 (5929) , 29-33
- https://doi.org/10.1038/305029a0
Abstract
Complementary DNA clones that code for the immunogenic region of the Plasmodium knowlesi circumsporozoite protein were shown to contain a tandemly repeating 36-base pair unit. A synthetic dodecapeptide corresponding to the predicted reading frame of the repeating nucleotide unit behaved, in an immunoradiometric assay, identically with the native P. knowlesi circumsporozoite protein. The repeating 36-base pair unit occurred 12 times within the gene and accounts for at least one-third of the amino acid sequence of the surface antigen protein.This publication has 19 references indexed in Scilit:
- Localization of a Plasmodium Surface Antigen Epitope by Tn5 Mutagenesis Mapping of a Recombinant cDNA CloneScience, 1983
- Circumsporozoite proteins of malaria parasites contain a single immunodominant region with two or more identical epitopes.The Journal of Experimental Medicine, 1983
- Cloning and expression in E. coli of the malarial sporozoite surface antigen gene from Plasmodium knowlesiNature, 1983
- A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragmentsGene, 1982
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Monoclonal antibodies to circumsporozoite proteins identify the species of malaria parasite in infected mosquitoesNature, 1982
- Cloning and sequence analysis reveal structural variation among related zein genes in maizeCell, 1982
- E. coli lactose operon ribosome binding siteNature, 1974
- Chemical Synthesis of Peptides and ProteinsAnnual Review of Biochemistry, 1970
- Solid Phase Peptide Synthesis. I. The Synthesis of a TetrapeptideJournal of the American Chemical Society, 1963